Plasmid Preparation:Article Title: A Pseudomonas aeruginosa endolytic muramidase targets cell-wall peptidoglycan in bacterial competition
Article Snippet: pEX18Ap Gene replacement vector with multiple-cloning site from pUC18; Apr (2) pEX18Ap-pseM pseM deletion plasmid, pEX18Ap with upstream and downstream regions of pseM This study pEX18Ap-hxcR hxcR deletion plasmid, pEX18Ap with upstream and downstream regions of hxcR This study pAK1900 E. coli-P. aeruginosa shuttle cloning vector carrying plac upstream of MCS; Apr, Cbr (3) pAK-retS pAK1900 with the entire retS gene This Lab pAK-rsmA pAK1900 with the entire rsmA gene This Lab pAK-pseM pAK1900 with the entire pseM gene This study pAK-pseMM pAK1900 with the entire pseMM gene This study pET28a-pseM The entire gene of pseM cloned into pET28a vector This study pET28a-pseMΔSS Protein expression construct, pseM lacking the signal peptide cloned into pET28a vector This study pET28a-pseMM Protein expression construct, pseMY36A/R90A/E120A/R128A cloned into pET28a vector This study pET22b T7 lac promoter-operator, carrying an N-terminal PelB signal sequence, Apr Novagen pET22b-pseM The entire gene of pseM cloned into pET22b vector This study pET22b-pseMΔSS Protein expression construct, pseM lacking the signal sequence cloned into pET22b vector This study pET-Duet-1 Dual T7 lac promoter-operator, N-terminal His tag, Apr Novagen pETDuet-pseM Protein expression construct, the entire gene of pseM This study cloned into the MCS1 of pET-Duet-1 vector pETDuet-pseM-PA0990 Protein expression construct, the entire gene of pseM and PA0990 cloned into the MCS1 and MCS2 of pETDuet-1 vector, respectively This study pBBR1-MCS5 Broad-Host-Range vector, Gmr (4) pMMB67HE-PA0990VSV pMMB67HE containing the entire PA0990 gene and the VSV sequence This Study mini-CTX-lacZ-Flag Integration plasmid, mini-CTX-lacZ containing the 3x flag sequence, Tcr This Lab (5) Mini-CTX-hcp1-Flag Mini-CTX-lacZ containing the entire hcp1 gene and the 3x flag sequence This Lab mini-CTX-pseM-Flag mini-CTX-lacZ containing the entire pseM gene This Study mini-CTX-pseMΔSS-Flag mini-CTX-lacZ containing the entire pseMΔSS gene This Study Table S3.
Cloning:Article Title: A Pseudomonas aeruginosa endolytic muramidase targets cell-wall peptidoglycan in bacterial competition
Article Snippet: pEX18Ap Gene replacement vector with multiple-cloning site from pUC18; Apr (2) pEX18Ap-pseM pseM deletion plasmid, pEX18Ap with upstream and downstream regions of pseM This study pEX18Ap-hxcR hxcR deletion plasmid, pEX18Ap with upstream and downstream regions of hxcR This study pAK1900 E. coli-P. aeruginosa shuttle cloning vector carrying plac upstream of MCS; Apr, Cbr (3) pAK-retS pAK1900 with the entire retS gene This Lab pAK-rsmA pAK1900 with the entire rsmA gene This Lab pAK-pseM pAK1900 with the entire pseM gene This study pAK-pseMM pAK1900 with the entire pseMM gene This study pET28a-pseM The entire gene of pseM cloned into pET28a vector This study pET28a-pseMΔSS Protein expression construct, pseM lacking the signal peptide cloned into pET28a vector This study pET28a-pseMM Protein expression construct, pseMY36A/R90A/E120A/R128A cloned into pET28a vector This study pET22b T7 lac promoter-operator, carrying an N-terminal PelB signal sequence, Apr Novagen pET22b-pseM The entire gene of pseM cloned into pET22b vector This study pET22b-pseMΔSS Protein expression construct, pseM lacking the signal sequence cloned into pET22b vector This study pET-Duet-1 Dual T7 lac promoter-operator, N-terminal His tag, Apr Novagen pETDuet-pseM Protein expression construct, the entire gene of pseM This study cloned into the MCS1 of pET-Duet-1 vector pETDuet-pseM-PA0990 Protein expression construct, the entire gene of pseM and PA0990 cloned into the MCS1 and MCS2 of pETDuet-1 vector, respectively This study pBBR1-MCS5 Broad-Host-Range vector, Gmr (4) pMMB67HE-PA0990VSV pMMB67HE containing the entire PA0990 gene and the VSV sequence This Study mini-CTX-lacZ-Flag Integration plasmid, mini-CTX-lacZ containing the 3x flag sequence, Tcr This Lab (5) Mini-CTX-hcp1-Flag Mini-CTX-lacZ containing the entire hcp1 gene and the 3x flag sequence This Lab mini-CTX-pseM-Flag mini-CTX-lacZ containing the entire pseM gene This Study mini-CTX-pseMΔSS-Flag mini-CTX-lacZ containing the entire pseMΔSS gene This Study Table S3.
Clone Assay:Article Title: A Pseudomonas aeruginosa endolytic muramidase targets cell-wall peptidoglycan in bacterial competition
Article Snippet: pEX18Ap Gene replacement vector with multiple-cloning site from pUC18; Apr (2) pEX18Ap-pseM pseM deletion plasmid, pEX18Ap with upstream and downstream regions of pseM This study pEX18Ap-hxcR hxcR deletion plasmid, pEX18Ap with upstream and downstream regions of hxcR This study pAK1900 E. coli-P. aeruginosa shuttle cloning vector carrying plac upstream of MCS; Apr, Cbr (3) pAK-retS pAK1900 with the entire retS gene This Lab pAK-rsmA pAK1900 with the entire rsmA gene This Lab pAK-pseM pAK1900 with the entire pseM gene This study pAK-pseMM pAK1900 with the entire pseMM gene This study pET28a-pseM The entire gene of pseM cloned into pET28a vector This study pET28a-pseMΔSS Protein expression construct, pseM lacking the signal peptide cloned into pET28a vector This study pET28a-pseMM Protein expression construct, pseMY36A/R90A/E120A/R128A cloned into pET28a vector This study pET22b T7 lac promoter-operator, carrying an N-terminal PelB signal sequence, Apr Novagen pET22b-pseM The entire gene of pseM cloned into pET22b vector This study pET22b-pseMΔSS Protein expression construct, pseM lacking the signal sequence cloned into pET22b vector This study pET-Duet-1 Dual T7 lac promoter-operator, N-terminal His tag, Apr Novagen pETDuet-pseM Protein expression construct, the entire gene of pseM This study cloned into the MCS1 of pET-Duet-1 vector pETDuet-pseM-PA0990 Protein expression construct, the entire gene of pseM and PA0990 cloned into the MCS1 and MCS2 of pETDuet-1 vector, respectively This study pBBR1-MCS5 Broad-Host-Range vector, Gmr (4) pMMB67HE-PA0990VSV pMMB67HE containing the entire PA0990 gene and the VSV sequence This Study mini-CTX-lacZ-Flag Integration plasmid, mini-CTX-lacZ containing the 3x flag sequence, Tcr This Lab (5) Mini-CTX-hcp1-Flag Mini-CTX-lacZ containing the entire hcp1 gene and the 3x flag sequence This Lab mini-CTX-pseM-Flag mini-CTX-lacZ containing the entire pseM gene This Study mini-CTX-pseMΔSS-Flag mini-CTX-lacZ containing the entire pseMΔSS gene This Study Table S3.
Expressing:Article Title: A Pseudomonas aeruginosa endolytic muramidase targets cell-wall peptidoglycan in bacterial competition
Article Snippet: pEX18Ap Gene replacement vector with multiple-cloning site from pUC18; Apr (2) pEX18Ap-pseM pseM deletion plasmid, pEX18Ap with upstream and downstream regions of pseM This study pEX18Ap-hxcR hxcR deletion plasmid, pEX18Ap with upstream and downstream regions of hxcR This study pAK1900 E. coli-P. aeruginosa shuttle cloning vector carrying plac upstream of MCS; Apr, Cbr (3) pAK-retS pAK1900 with the entire retS gene This Lab pAK-rsmA pAK1900 with the entire rsmA gene This Lab pAK-pseM pAK1900 with the entire pseM gene This study pAK-pseMM pAK1900 with the entire pseMM gene This study pET28a-pseM The entire gene of pseM cloned into pET28a vector This study pET28a-pseMΔSS Protein expression construct, pseM lacking the signal peptide cloned into pET28a vector This study pET28a-pseMM Protein expression construct, pseMY36A/R90A/E120A/R128A cloned into pET28a vector This study pET22b T7 lac promoter-operator, carrying an N-terminal PelB signal sequence, Apr Novagen pET22b-pseM The entire gene of pseM cloned into pET22b vector This study pET22b-pseMΔSS Protein expression construct, pseM lacking the signal sequence cloned into pET22b vector This study pET-Duet-1 Dual T7 lac promoter-operator, N-terminal His tag, Apr Novagen pETDuet-pseM Protein expression construct, the entire gene of pseM This study cloned into the MCS1 of pET-Duet-1 vector pETDuet-pseM-PA0990 Protein expression construct, the entire gene of pseM and PA0990 cloned into the MCS1 and MCS2 of pETDuet-1 vector, respectively This study pBBR1-MCS5 Broad-Host-Range vector, Gmr (4) pMMB67HE-PA0990VSV pMMB67HE containing the entire PA0990 gene and the VSV sequence This Study mini-CTX-lacZ-Flag Integration plasmid, mini-CTX-lacZ containing the 3x flag sequence, Tcr This Lab (5) Mini-CTX-hcp1-Flag Mini-CTX-lacZ containing the entire hcp1 gene and the 3x flag sequence This Lab mini-CTX-pseM-Flag mini-CTX-lacZ containing the entire pseM gene This Study mini-CTX-pseMΔSS-Flag mini-CTX-lacZ containing the entire pseMΔSS gene This Study Table S3.
Construct:Article Title: A Pseudomonas aeruginosa endolytic muramidase targets cell-wall peptidoglycan in bacterial competition
Article Snippet: pEX18Ap Gene replacement vector with multiple-cloning site from pUC18; Apr (2) pEX18Ap-pseM pseM deletion plasmid, pEX18Ap with upstream and downstream regions of pseM This study pEX18Ap-hxcR hxcR deletion plasmid, pEX18Ap with upstream and downstream regions of hxcR This study pAK1900 E. coli-P. aeruginosa shuttle cloning vector carrying plac upstream of MCS; Apr, Cbr (3) pAK-retS pAK1900 with the entire retS gene This Lab pAK-rsmA pAK1900 with the entire rsmA gene This Lab pAK-pseM pAK1900 with the entire pseM gene This study pAK-pseMM pAK1900 with the entire pseMM gene This study pET28a-pseM The entire gene of pseM cloned into pET28a vector This study pET28a-pseMΔSS Protein expression construct, pseM lacking the signal peptide cloned into pET28a vector This study pET28a-pseMM Protein expression construct, pseMY36A/R90A/E120A/R128A cloned into pET28a vector This study pET22b T7 lac promoter-operator, carrying an N-terminal PelB signal sequence, Apr Novagen pET22b-pseM The entire gene of pseM cloned into pET22b vector This study pET22b-pseMΔSS Protein expression construct, pseM lacking the signal sequence cloned into pET22b vector This study pET-Duet-1 Dual T7 lac promoter-operator, N-terminal His tag, Apr Novagen pETDuet-pseM Protein expression construct, the entire gene of pseM This study cloned into the MCS1 of pET-Duet-1 vector pETDuet-pseM-PA0990 Protein expression construct, the entire gene of pseM and PA0990 cloned into the MCS1 and MCS2 of pETDuet-1 vector, respectively This study pBBR1-MCS5 Broad-Host-Range vector, Gmr (4) pMMB67HE-PA0990VSV pMMB67HE containing the entire PA0990 gene and the VSV sequence This Study mini-CTX-lacZ-Flag Integration plasmid, mini-CTX-lacZ containing the 3x flag sequence, Tcr This Lab (5) Mini-CTX-hcp1-Flag Mini-CTX-lacZ containing the entire hcp1 gene and the 3x flag sequence This Lab mini-CTX-pseM-Flag mini-CTX-lacZ containing the entire pseM gene This Study mini-CTX-pseMΔSS-Flag mini-CTX-lacZ containing the entire pseMΔSS gene This Study Table S3.
Sequencing:Article Title: A Pseudomonas aeruginosa endolytic muramidase targets cell-wall peptidoglycan in bacterial competition
Article Snippet: pEX18Ap Gene replacement vector with multiple-cloning site from pUC18; Apr (2) pEX18Ap-pseM pseM deletion plasmid, pEX18Ap with upstream and downstream regions of pseM This study pEX18Ap-hxcR hxcR deletion plasmid, pEX18Ap with upstream and downstream regions of hxcR This study pAK1900 E. coli-P. aeruginosa shuttle cloning vector carrying plac upstream of MCS; Apr, Cbr (3) pAK-retS pAK1900 with the entire retS gene This Lab pAK-rsmA pAK1900 with the entire rsmA gene This Lab pAK-pseM pAK1900 with the entire pseM gene This study pAK-pseMM pAK1900 with the entire pseMM gene This study pET28a-pseM The entire gene of pseM cloned into pET28a vector This study pET28a-pseMΔSS Protein expression construct, pseM lacking the signal peptide cloned into pET28a vector This study pET28a-pseMM Protein expression construct, pseMY36A/R90A/E120A/R128A cloned into pET28a vector This study pET22b T7 lac promoter-operator, carrying an N-terminal PelB signal sequence, Apr Novagen pET22b-pseM The entire gene of pseM cloned into pET22b vector This study pET22b-pseMΔSS Protein expression construct, pseM lacking the signal sequence cloned into pET22b vector This study pET-Duet-1 Dual T7 lac promoter-operator, N-terminal His tag, Apr Novagen pETDuet-pseM Protein expression construct, the entire gene of pseM This study cloned into the MCS1 of pET-Duet-1 vector pETDuet-pseM-PA0990 Protein expression construct, the entire gene of pseM and PA0990 cloned into the MCS1 and MCS2 of pETDuet-1 vector, respectively This study pBBR1-MCS5 Broad-Host-Range vector, Gmr (4) pMMB67HE-PA0990VSV pMMB67HE containing the entire PA0990 gene and the VSV sequence This Study mini-CTX-lacZ-Flag Integration plasmid, mini-CTX-lacZ containing the 3x flag sequence, Tcr This Lab (5) Mini-CTX-hcp1-Flag Mini-CTX-lacZ containing the entire hcp1 gene and the 3x flag sequence This Lab mini-CTX-pseM-Flag mini-CTX-lacZ containing the entire pseM gene This Study mini-CTX-pseMΔSS-Flag mini-CTX-lacZ containing the entire pseMΔSS gene This Study Table S3.
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